EGFP and GFP Fluorescent Protein Plasmids

EGFP (enhanced Green Fluorescent Protein) and GFP-based fluorescent proteins are widely used reporter genes in molecular biology and cell biology to visualize gene expression, protein localization, and cellular processes in live cells.

Regenitus EGFP and GFP plasmids are designed for mammalian expression systems, enabling robust fluorescence in a wide range of cell types under common promoters such as CMV.

These vectors are commonly used for:

Monitoring transfection efficiency
Live-cell imaging and protein localization studies
Gene expression reporting systems
Fusion protein tagging (N-terminal or C-terminal)
Stable and transient mammalian expression assays

EGFP offers improved brightness and photostability compared to wild-type GFP, making it the preferred choice for most modern fluorescence applications.

Vector Features (typical constructs include)

Strong mammalian promoters (e.g., CMV or EF1α)
High-expression EGFP coding sequence
Bacterial selection marker for plasmid propagation
Mammalian backbone optimized for transient expression
Compatible multiple cloning sites (MCS)

Choosing between GFP and EGFP

EGFP is an optimized variant of GFP with improved fluorescence intensity and reduced temperature sensitivity. It is generally preferred for mammalian expression systems due to higher signal output and stability.