CMV-EGFP Mammalian Expression Plasmid (pReg-CMV-EGFP-v1)

This vector is designed for robust constitutive expression of Enhanced Green Fluorescent Protein (EGFP) in a wide range of mammalian cell types. Expression of EGFP is driven by the human cytomegalovirus (CMV) immediate-early promoter, providing high-level transgene expression in commonly used cell lines including HEK293, HeLa, CHO, COS-7, and many primary cell types.

EGFP serves as a convenient fluorescent reporter for monitoring transfection efficiency, gene expression, promoter activity, and cell tracking experiments. The bright green fluorescence can be readily detected by fluorescence microscopy, flow cytometry, and other fluorescence-based assays.

This plasmid is suitable for transient transfection studies, stable cell line generation, optimization of transfection protocols, and general molecular and cellular biology applications.

The CMV-EGFP plasmid backbone contains ampicillin resistance for bacterial selection before transfection and neomycin resistance gene for geneticin (G418) selection of stably transfected eukaryotic cells.

Total vector size 6107 bp.

Research Use Only (RUO).

Applications

• Mammalian cell transfection
• EGFP fusion protein expression
• Fluorescence imaging
• Protein localization studies
• Live-cell microscopy
• Gene expression analysis
• Cell biology research
• Molecular cloning

Features

• Strong CMV promoter for high-level expression
• Enhanced Green Fluorescent Protein (EGFP) reporter
• Suitable for transient and stable expression studies
• Ideal for imaging and localization experiments