EGFP-N1 Mammalian Expression Plasmid (pReg-CMV-EGFP-N1-v4)

This vector is designed for robust constitutive expression of Enhanced Green Fluorescent Protein (EGFP) in a wide range of mammalian cell types.

EGFP expression is driven by the human cytomegalovirus (CMV) immediate-early promoter.

The multiple cloning site (MCS) is located at the N-terminus. This enables the generation of C-terminal EGFP fusion proteins, allowing researchers to monitor protein expression, intracellular localization, and cellular dynamics using fluorescence microscopy and live-cell imaging.

Plasmid contains kanamycin resistance for bacterial selection and neomycin resistance for stable selection with G418 in eukaryotic cells.

Ideal for transient and stable transfection experiments in a broad range of mammalian cell lines.

Total vector size is 4732 bp.

Research Use Only (RUO).

Applications

• Mammalian gene expression
• C-terminal fluorescent protein tagging
• Protein localization studies
• Fluorescence microscopy
• Live-cell imaging
• Cell biology research
• Molecular cloning
• Protein trafficking analysis
• Functional protein characterization
• Transfection optimization

Features

• Strong CMV immediate-early promoter
• Designed for C-terminal EGFP fusion protein expression
• Bright Enhanced Green Fluorescent Protein (EGFP)
• High expression in mammalian cells
• Suitable for transient and stable transfection
• Compatible with common mammalian cell lines
• Ideal for fluorescence microscopy and live-cell imaging