Red Fluorescent Protein (RFP) Plasmids — DsRed Vectors

Red fluorescent proteins (RFPs) are widely used reporter proteins in molecular and cell biology for visualizing gene expression, monitoring protein localization, and tracking cellular processes in live mammalian cells. Their longer emission wavelength compared with green fluorescent proteins reduces background autofluorescence and makes them well suited for multicolor fluorescence imaging.

This collection contains DsRed-based mammalian expression plasmids designed for research applications. DsRed was one of the first widely adopted red fluorescent proteins and remains a valuable reporter for fluorescence microscopy, transfection experiments, and dual-label imaging studies.

Applications

DsRed plasmids are commonly used for:

  • Live-cell fluorescence imaging
  • Monitoring transfection efficiency
  • Protein localization studies
  • Reporter gene assays
  • Co-expression experiments with GFP or YFP vectors
  • Dual-color fluorescence microscopy

About DsRed

DsRed is a red fluorescent protein originally derived from coral and engineered for use in mammalian cells. It produces bright red fluorescence and has been widely used as a reporter gene in molecular biology.

Researchers often choose DsRed for experiments requiring clear spectral separation from GFP or YFP, making it useful in dual-label and multicolor imaging applications.


Typical Vector Features

Plasmids in this collection typically include:

  • Strong mammalian promoters (such as CMV or EF1α)
  • Optimized DsRed coding sequence
  • Multiple cloning site (MCS)
  • Bacterial antibiotic resistance marker
  • Mammalian expression backbone suitable for transient transfection

Individual plasmids may include additional features described on their respective product pages.


Why Choose Red Fluorescent Proteins?

Compared with green fluorescent proteins, red fluorescent proteins offer several advantages:

  • Reduced overlap with GFP fluorescence
  • Improved multicolor imaging capability
  • Lower cellular autofluorescence in many tissues
  • Excellent compatibility with GFP and YFP reporter systems


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