EF1α-DsRed-C1 Mammalian Expression Plasmid (pReg-EF1α-DsRed-C1-v6)

This vector is a mammalian expression plasmid designed for robust transgene expression in a wide range of mammalian cell types, including hard-to-transfect cell lines, stem cells, and primary cultures.

It contains red fluorescent protein - DsRed- driven by human elongation factor-1 alpha (EF1α) constitutive promoter for high, stable, long-term transgene expression in mammalian cells. 

The multiple cloning site (MCS) is located at C terminus in frame with dsRed. This enables the generation of N-terminal DsRed fusion proteins.

Plasmid carries ampicillin resistance for bacterial selection and neomycin resistance for stable selection with G418 in eukaryotic cells.

Total vector size 6460 bp.

Research Use Only (RUO).

Applications

• Generation of N-terminal DsRed fusion proteins
• Protein localization studies
• Live-cell fluorescence imaging
• Subcellular trafficking analysis
• Flow cytometry
• Suitable for transient and stable expression studies

Features

• Strong EF1α promoter for constitutive expression
• N-terminal DsRed fusion design
• Suitable for a broad range of mammalian cell types
• Sequence-verified construct