Blue and Cyan Fluorescent Protein Plasmids – EBFP and ECFP
Blue fluorescent protein (BFP) and cyan fluorescent protein (CFP) reporters are engineered derivatives of green fluorescent protein (GFP) that emit fluorescence at shorter wavelengths. They are widely used in molecular biology, cell biology, and biotechnology to visualize gene expression, monitor protein localization, and perform multicolor fluorescence imaging.
This collection includes mammalian expression plasmids encoding blue and cyan fluorescent proteins, including EBFP (Enhanced Blue Fluorescent Protein) and ECFP (Enhanced Cyan Fluorescent Protein). These reporters are commonly used in combination with green, yellow, and red fluorescent proteins to enable simultaneous visualization of multiple cellular targets.
Applications
These plasmids are suitable for:
- Live-cell fluorescence imaging
- Monitoring transfection efficiency
- Protein localization studies
- Multicolor fluorescence microscopy
- FRET (Förster Resonance Energy Transfer) experiments
- Reporter gene assays
Fluorescent Protein Variants
EBFP (Enhanced Blue Fluorescent Protein) provides bright blue fluorescence and is useful for multicolor imaging when combined with green, yellow, or red fluorescent proteins.
ECFP (Enhanced Cyan Fluorescent Protein) emits cyan fluorescence and is frequently used in FRET-based biosensors and protein interaction studies due to its spectral compatibility with YFP variants.
Typical Vector Features
Plasmids in this collection typically include:
- Strong mammalian promoters (e.g., CMV or EF1α)
- Optimized fluorescent protein coding sequences
- Multiple cloning site (MCS)
- Bacterial antibiotic resistance marker
- Mammalian expression backbone
Related Collections
- EGFP & GFP Fluorescent Protein Plasmids
- Yellow Fluorescent Protein Plasmids
- Red Fluorescent Protein Plasmids
- LacZ Reporter Plasmids
