EF1α-EGFP-C1 Mammalian Expression Plasmid (pReg-EF1α-EGFP-C1-v7)

This vector is a mammalian expression plasmid  designed for robust transgene expression in a wide range of mammalian cell types, including hard-to-transfect cell lines, stem cells, and primary cultures.

It contains the enhanced green fluorescent protein (EGFP) driven by human elongation factor-1 alpha (EF1α) constitutive promoter for high, stable, long-term transgene expression in mammalian cells.

The multiple cloning site (MCS) is located at the C-terminus in frame with EGFP. This enables the generation of N-terminal EGFP fusion proteins.

Plasmid contains ampicillin resistance for bacterial selection and neomycin resistance for stable selection with G418 in eukaryotic cells.

Total vector size 6499 bp.

Research Use Only (RUO)

Features

• Strong EF1α promoter for high-level expression
• Enhanced Green Fluorescent Protein (EGFP) reporter
• Suitable for transient and stable expression studies
• Ideal for imaging and localization experiments

Applications

• Generation of N-terminal EGFP fusion proteins
• Protein localization studies
• Live-cell fluorescence imaging
• Subcellular trafficking analysis
• Flow cytometry
• Stable cell line generation