EF1α-EGFP-C1 Mammalian Expression Plasmid (pReg-EF1α-EGFP-C1-v8)
5µg research-grade DNA
$169.00
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EF1α-EGFP-C1 Mammalian Expression Plasmid (pReg-EF1α-EGFP-C1-v8)
Catalog ID: pReg-EF1a-EGFP-C1-v8
$169.00
This vector is a mammalian expression plasmid designed for robust transgene expression in a wide range of mammalian cell types, including hard-to-transfect cell lines, stem cells, and primary cultures.
It contains the enhanced green fluorescent protein (EGFP) driven by human elongation factor-1 alpha (EF1α) constitutive promoter for high, stable, long-term transgene expression in mammalian cells.
The multiple cloning site (MCS) is located at the C-terminus. This enables the generation of N-terminal EGFP fusion proteins.
Plasmid contains ampicillin resistance for bacterial selection and neomycin resistance for stable selection with G418 in eukaryotic cells.
Total vector size 6477 bp.
Research Use Only (RUO)
Applications
- Generation of N-terminal EGFP fusion proteins
- Protein localization studies
- Live-cell fluorescence imaging
- Subcellular trafficking analysis
- Flow cytometry
- Stable cell line generation
- Mammalian gene expression studies
Features
- Strong EF1α promoter for high-level expression
- Enhanced Green Fluorescent Protein (EGFP) reporter
- Suitable for transient and stable expression studies
- Ideal for imaging and localization experiments