CMV-EGFP-N1 Mammalian Expression Plasmid (pReg-CMV-EGFP-N1-v2)

This vector is a mammalian expression plasmid designed for constitutive expression of proteins fused to the N-terminus of Enhanced Green Fluorescent Protein (EGFP) in mammalian cells.

Expression is driven by the human cytomegalovirus (CMV) immediate-early promoter, providing robust transgene expression in a broad range of mammalian cell lines.

This vector enables cloning of a gene of interest upstream of the EGFP coding sequence, resulting in expression of a protein with a C-terminal EGFP tag.

Fluorescent fusion proteins are widely used to investigate protein localization, intracellular trafficking, expression levels, and protein dynamics in living cells.

The vector is suitable for transient transfection, stable cell line generation, fluorescence microscopy, flow cytometry, and other fluorescence-based applications. It is compatible with standard molecular cloning workflows and is intended for research applications requiring fluorescent protein tagging.

Plasmid carries ampicillin resistance for bacterial selection and neomycin resistance gene for geneticin (G418) selection of stably transfected eukaryotic cells.

Total vector size 6124 bp.

Research Use Only (RUO)

Applications

• Generation of C-terminal EGFP fusion proteins
• Protein localization studies
• Live-cell fluorescence imaging
• Subcellular trafficking analysis
• Flow cytometry
• Stable cell line generation
• Mammalian gene expression studies

Features

• Strong CMV promoter for constitutive expression
• C-terminal EGFP fusion design
• Bright Enhanced Green Fluorescent Protein (EGFP)
• Suitable for a broad range of mammalian cell types
• Sequence-verified construct