CMV-EBFP-N1 Mammalian Expression Plasmid (pReg-CMV-EBFP-N1-v2)
5µg research-grade DNA
$169.00
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CMV-EBFP-N1 Mammalian Expression Plasmid (pReg-CMV-EBFP-N1-v2)
Catalog ID: pReg-CMV-EBFP-N1-v2
$169.00
This vector contains enhanced blue fluorescent protein - EBFP- driven by cytomegalovirus (CMV) promoter for high level expression in mammalian cells.
The multiple cloning site (MCS) is located at N terminus. This enables the generation of C-terminal EBFP fusion proteins, allowing researchers to monitor protein expression, intracellular localization, and cellular dynamics using fluorescence microscopy and live-cell imaging.
Plasmid carries ampicillin resistance for bacterial selection and neomycin resistance for stable selection with G418 in eukaryotic cells.
Total vector size 6124 bp.
Research Use Only (RUO).
Applications
- Generation of C-terminal EBFP fusion proteins
- Protein localization studies
- Live-cell fluorescence imaging
- Subcellular trafficking analysis
- Flow cytometry
- Suitable for transient and stable expression studies
Features
- Strong CMV promoter for constitutive expression
- Bright Enhanced Blue Fluorescent Protein (EBFP)
- Suitable for a broad range of mammalian cell types
- Sequence-verified construct