{"product_id":"pcmv-eyfp-n1-v2-vector","title":"CMV-EYFP-N1 Mammalian Expression Plasmid (pReg-CMV-EYFP-N1-v2)","description":"\u003cp\u003e\u003cmeta charset=\"UTF-8\"\u003e\u003cstrong\u003eThis vector is a mammalian expression plasmid\u003cspan class=\"apple-converted-space\"\u003e \u003c\/span\u003edesigned for robust transgene expression in a wide range of mammalian cell types.\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIt contains the enhanced yellow fluorescent protein (EYFP) driven by cytomegalovirus (CMV) promoter for high level expression in mammalian cells.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe multiple cloning site (MCS) is located at the N-terminus. \u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eThis enables the generation of C-terminal EYFP fusion proteins.\u003c\/span\u003e\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cspan\u003ePlasmid carries ampicillin resistance for bacterial selection and neomycin resistance for stable selection with G418 in eukaryotic cells.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTotal vector size 6124 bp.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Use Only (RUO).\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch4 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h4\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\" style=\"font-weight: bold;\"\u003e\u003cstrong\u003eGeneration of C-terminal EYFP fusion proteins\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"font-weight: bold;\"\u003e\u003cstrong\u003eProtein localization studies\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"font-weight: bold;\"\u003e\u003cstrong\u003eLive-cell fluorescence imaging\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"font-weight: bold;\"\u003e\u003cstrong\u003eSubcellular trafficking analysis\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"font-weight: bold;\"\u003e\u003cstrong\u003eFlow cytometry\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"font-weight: bold;\"\u003e\u003cstrong\u003eSuitable for transient and stable expression studies\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4 class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eFeature\u003cmeta charset=\"UTF-8\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/h4\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\" style=\"font-weight: bold;\"\u003e\u003cstrong\u003eStrong CMV promoter for constitutive expression\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"font-weight: bold;\"\u003e\u003cstrong\u003eC-terminal EYFP fusion design\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"font-weight: bold;\"\u003e\u003cstrong\u003eSuitable for a broad range of mammalian cell types\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"font-weight: bold;\"\u003e\u003cstrong\u003eSequence-verified construct\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Regenitus Biosciences","offers":[{"title":"5µg research-grade DNA","offer_id":52606643503416,"sku":"pReg11-EYFP","price":167.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0979\/4617\/3752\/files\/pReg-CMV-EYFP-N1-v2Map.png?v=1771466593","url":"https:\/\/regenitus.com\/products\/pcmv-eyfp-n1-v2-vector","provider":"Regenitus Biosciences","version":"1.0","type":"link"}