{"product_id":"pcmv-egfp-n1-v2-vector","title":"CMV-EGFP-N1 Mammalian Expression Plasmid (pReg-CMV-EGFP-N1-v2)","description":"\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eThis vector is a mammalian expression plasmid designed for constitutive expression of proteins fused to the N-terminus of Enhanced Green Fluorescent Protein (EGFP) in mammalian cells.\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eExpression is driven by the human cytomegalovirus (CMV) immediate-early promoter, providing robust transgene expression in a broad range of mammalian cell lines.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"isSelectedEnd\"\u003e\u003cstrong\u003eThis vector enables cloning of a gene of interest upstream of the EGFP coding sequence, resulting in expression of a protein with a C-terminal EGFP tag. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"isSelectedEnd\"\u003e\u003cstrong\u003eFluorescent fusion proteins are widely used to investigate protein localization, intracellular trafficking, expression levels, and protein dynamics in living cells.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThe vector is suitable for transient transfection, stable cell line generation, fluorescence microscopy, flow cytometry, and other fluorescence-based applications. It is compatible with standard molecular cloning workflows and is intended for research applications requiring fluorescent protein tagging.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003ePlasmid carries ampicillin resistance for bacterial selection and neomycin resistance gene for geneticin (G418) selection of stably transfected eukaryotic cells\u003cspan data-copy-service-computed-style='font-family: \"Google Sans\", \"Helvetica Neue\", sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);'\u003e.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eTotal vector size 6124 bp.\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"isSelectedEnd\"\u003e \u003c\/p\u003e\n\u003ch4 class=\"isSelectedEnd\"\u003e\u003cstrong\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cul data-spread=\"false\"\u003e\n\u003cli\u003e\u003cspan\u003eGeneration of C-terminal EGFP fusion proteins\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eProtein localization studies\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eLive-cell fluorescence imaging\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSubcellular trafficking analysis\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eFlow cytometry\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eStable cell line generation\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eMammalian gene expression studies\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4\u003e\u003cstrong\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h4\u003e\n\u003cul data-spread=\"false\"\u003e\n\u003cli\u003e\u003cspan\u003eStrong CMV promoter for constitutive expression\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eC-terminal EGFP fusion design\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBright Enhanced Green Fluorescent Protein (EGFP)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSuitable for a broad range of mammalian cell types\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eSequence-verified construct\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Regenitus Biosciences","offers":[{"title":"5µg research-grade DNA","offer_id":52606644584760,"sku":"Reg002","price":149.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0979\/4617\/3752\/files\/pReg-CMV-EGFP-N1-v2_c3d5e34d-42ce-4906-9ecf-ac92a626e9cc.png?v=1770599540","url":"https:\/\/regenitus.com\/products\/pcmv-egfp-n1-v2-vector","provider":"Regenitus Biosciences","version":"1.0","type":"link"}