{"product_id":"cmv-egfp-mammalian-expression-vector","title":"CMV-EGFP Mammalian Expression Plasmid (pReg-CMV-EGFP-v1)","description":"\u003cp\u003e\u003cstrong\u003eThis vector is designed for robust constitutive expression of Enhanced Green Fluorescent Protein (EGFP) in a wide range of mammalian cell types. Expression of EGFP is driven by the human cytomegalovirus (CMV) immediate-early promoter, providing high-level transgene expression in commonly used cell lines including HEK293, HeLa, CHO, COS-7, and many primary cell types.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"isselectedend\"\u003e\u003cstrong\u003eEGFP serves as a convenient fluorescent reporter for monitoring transfection efficiency, gene expression, promoter activity, and cell tracking experiments. The bright green fluorescence can be readily detected by fluorescence microscopy, flow cytometry, and other fluorescence-based assays.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThis plasmid is suitable for transient transfection studies, stable cell line generation, optimization of transfection protocols, and general molecular and cellular biology applications. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eThe CMV-EGFP plasmid backbone contains ampicillin resistance for bacterial selection before transfection and neomycin resistance gene for geneticin (G418) selection of stably transfected eukaryotic cells\u003cspan data-copy-service-computed-style='font-family: \"Google Sans\", \"Helvetica Neue\", sans-serif; font-size: 16px; font-weight: 400; margin: 0px; text-decoration: none; border-bottom: 0px rgb(10, 10, 10);'\u003e.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTotal vector size 6107 bp.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Use Only (RUO).\u003cmeta charset=\"UTF-8\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch4 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h4\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eMammalian cell transfection\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eEGFP fusion protein expression\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eFluorescence imaging\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eProtein localization studies\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eLive-cell microscopy\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eGene expression analysis\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eCell biology research\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eMolecular cloning\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h4\u003e\n\u003cul type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eStrong CMV promoter for high-level expression\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eEnhanced Green Fluorescent Protein (EGFP) reporter\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eSuitable for transient and stable expression studies\u003c\/span\u003e\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\u003cspan\u003eIdeal for imaging and localization experiments\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Regenitus Biosciences","offers":[{"title":"5µg research-grade DNA","offer_id":52606644551992,"sku":"Reg001","price":149.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0979\/4617\/3752\/files\/pReg-CMV-EGFP-v1.png?v=1770515344","url":"https:\/\/regenitus.com\/products\/cmv-egfp-mammalian-expression-vector","provider":"Regenitus Biosciences","version":"1.0","type":"link"}